Mouse SOD2(Superoxide Dismutase 2, Mitochondrial) ELISA Kit


Mouse SOD2(Superoxide Dismutase 2, Mitochondrial) ELISA Kit

Cat No:HR1E8612
Price:$399/96T
Product available

  Product info

Alternative Name: IPO-B; MNSOD; Mn-SOD

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 2, Mitochondrial(SOD2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Superoxide Dismutase 2, Mitochondrial(SOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 2, Mitochondrial(SOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Superoxide Dismutase 2, Mitochondrial(SOD2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse SOD2(Superoxide Dismutase 2, Mitochondrial) ELISA Kit
Cat NoHR1E8612
SpeciesMouse
Alternative NameIPO-B; MNSOD; Mn-SOD
Sensitivity14.5 pg/mL
Standard2000 pg/mL
Detection Range31.25-2000 pg/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science;
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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