Dog DSG1(Desmoglein 1) ELISA Kit


Dog DSG1(Desmoglein 1) ELISA Kit

Cat No:HR1E8625
Price:$399/96T
Product available

  Product info

Alternative Name: CDHF4; DG1; DSG; Cadherin family member 4; Desmosomal glycoprotein 1; Pemphigus foliaceus antigen

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoglein 1(DSG1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Desmoglein 1(DSG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoglein 1(DSG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Desmoglein 1(DSG1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Dog DSG1(Desmoglein 1) ELISA Kit
Cat NoHR1E8625
SpeciesDog
Alternative NameCDHF4; DG1; DSG; Cadherin family member 4; Desmosomal glycoprotein 1; Pemphigus foliaceus antigen
Sensitivity0.56 ng/mL
Standard100 ng/mL
Detection Range1.57-100 ng/mL
Assay Length3.5h
Research AreaCD & Adhesion molecule;Dermatology;Autoimmunity;
Sample Typetissue homogenates, cell lysates and other biological fluids

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