Mouse COL2a1(Collagen Type II Alpha 1) ELISA Kit


Mouse COL2a1(Collagen Type II Alpha 1) ELISA Kit

Cat No:HR1E8649
Price:$399/96T
Product available

  Product info

Alternative Name: COL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II Alpha 1(COL2a1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type II Alpha 1(COL2a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II Alpha 1(COL2a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Collagen Type II Alpha 1(COL2a1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse COL2a1(Collagen Type II Alpha 1) ELISA Kit
Cat NoHR1E8649
SpeciesMouse
Alternative NameCOL2-A1; COL2A1; SEDC; Chondrocalcin; Collagen Alpha-1(II)Chain; Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital
Sensitivity0.059 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/L
Assay Length3.5h
Research AreaMetabolic pathway;
Sample TypeSerum, plasma and other biological fluids.

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