Rat Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit


Rat Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit

Cat No:HR1E8658
Price:$399/96T
Product available

  Product info

Alternative Name: MADH5; Dwfc; JV5-1

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 5(Smad5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 5(Smad5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 5(Smad5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mothers Against Decapentaplegic Homolog 5(Smad5) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit
Cat NoHR1E8658
SpeciesRat
Alternative NameMADH5; Dwfc; JV5-1
Sensitivity0.123 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Hematology;Bone metabolism;
Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids

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