Avian bHB(Beta-Hydroxybutyric Acid) ELISA Kit

Avian bHB(Beta-Hydroxybutyric Acid) ELISA Kit

Cat No:HR1E8710
Product available

  Product info

Alternative Name: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid

Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Beta-Hydroxybutyric Acid(bHB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-Hydroxybutyric Acid(bHB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Beta-Hydroxybutyric Acid(bHB) in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Details

Product Name Avian bHB(Beta-Hydroxybutyric Acid) ELISA Kit
Cat NoHR1E8710
Alternative NameOHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Sensitivity0.48 ?g/mL
Standard100 ?g/mL
Detection Range1.57-100 ?g/mL
Assay Length2h
Research AreaSignal transduction;Metabolic pathway;Endocrinology;

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