Description:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SA(Salicylic Acid ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SA(Salicylic Acid ). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of SA(Salicylic Acid ) in the samples is then determined by comparing the OD of the samples to the standard curve.
