Avian 5-HT(5-Hydroxytryptamine) ELISA Kit

Avian 5-HT(5-Hydroxytryptamine) ELISA Kit

Cat No:HR1E8727
Product available

  Product info

Alternative Name: 5HT; Serotonin; N-Methyl-Gamma

Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 5-Hydroxytryptamine(5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Details

Product Name Avian 5-HT(5-Hydroxytryptamine) ELISA Kit
Cat NoHR1E8727
Alternative Name5HT; Serotonin; N-Methyl-Gamma
Sensitivity3.97 ng/mL
Standard900 ng/mL
Detection Range14.07-900 ng/mL
Assay Length2h
Research AreaSignal transduction;Infection immunity;Neuro science;Gastroenterology;

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