Cattle P?NP(Procollagen ? N-Terminal Propeptide) ELISA Kit


Cattle P?NP(Procollagen ? N-Terminal Propeptide) ELISA Kit

Cat No:HR1E8777
Price:$399/96T
Product available

  Product info

Alternative Name: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen ? N-Terminal Propeptide(Cattle P?NP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen ? N-Terminal Propeptide(Cattle P?NP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen ? N-Terminal Propeptide(Cattle P?NP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Procollagen ? N-Terminal Propeptide(Cattle P?NP) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Cattle P?NP(Procollagen ? N-Terminal Propeptide) ELISA Kit
Cat NoHR1E8777
SpeciesCattle
Alternative NameP1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide
Sensitivity1.19 ng/mL
Standard200 ng/mL
Detection Range3.13-200 ng/mL
Assay Length3.5h
Research AreaMetabolic pathway;Hepatology;Bone metabolism;
Sample Typeserum, plasma, tissue homogenates and other biological fluids

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