Rat TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit


Rat TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Cat No:HR1E8926
Price:$399/96T
Product available

  Product info

Alternative Name: TBARS; Thiobarbituric Acid Reactive Substance

Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thiobarbituric Acid Reactive Substance(TBARS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thiobarbituric Acid Reactive Substance(TBARS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Thiobarbituric Acid Reactive Substance(TBARS) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Cat NoHR1E8926
SpeciesRat
Alternative NameTBARS; Thiobarbituric Acid Reactive Substance
Sensitivity3.55 nmol/ml
Standard400nmol/ml
Detection Range6.25-400 nmol/ml
Assay Length2h
Research AreaMetabolic pathway;Hepatology;Hormone metabolism;
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

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