Human ACSL4(Acyl Coenzyme A Synthetase Long Chain Family, Member 4) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4(ACSL4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acyl Coenzyme A Synthetase Long Chain Family, Member 4(ACSL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acyl Coenzyme A Synthetase Long Chain Family, Member 4(ACSL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Acyl Coenzyme A Synthetase Long Chain Family, Member 4(ACSL4) in the samples is then determined by comparing the OD of the samples to the standard curve.

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