Human CNTF(Ciliary Neurotrophic Factor) ELISA Kit


Human CNTF(Ciliary Neurotrophic Factor) ELISA Kit

Cat No:HR1E1118
Price:$399/96T
Product available

  Product info

Alternative Name: HCNTF

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor(CNTF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor(CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ciliary Neurotrophic Factor(CNTF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Ciliary Neurotrophic Factor(CNTF) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human CNTF(Ciliary Neurotrophic Factor) ELISA Kit
Cat NoHR1E1118
SpeciesHuman
Alternative NameHCNTF
Sensitivity3.2 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaCytokine;Neuro science;

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