Human HRH3(Histamine Receptor H3) ELISA Kit


Human HRH3(Histamine Receptor H3) ELISA Kit

Cat No:HR1E9136
Price:$399/96T
Product available

  Product info

Alternative Name: GPCR97; HH3R; G-protein coupled receptor 97

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H3(HRH3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histamine Receptor H3(HRH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H3(HRH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Histamine Receptor H3(HRH3) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human HRH3(Histamine Receptor H3) ELISA Kit
Cat NoHR1E9136
SpeciesHuman
Alternative NameGPCR97; HH3R; G-protein coupled receptor 97
Sensitivity0.124 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaCD & Adhesion molecule;
Sample TypeTissue homogenates and other biological fluids.

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