Human CCA(Colon Cancer Antigen) ELISA Kit


Human CCA(Colon Cancer Antigen) ELISA Kit

Cat No:HR1E9174
Price:$399/96T
Product available

  Product info

Alternative Name: Colon Cancer Antigen

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colon Cancer Antigen(CCA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Colon Cancer Antigen(CCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colon Cancer Antigen(CCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Colon Cancer Antigen(CCA) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human CCA(Colon Cancer Antigen) ELISA Kit
Cat NoHR1E9174
SpeciesHuman
Alternative NameColon Cancer Antigen
Sensitivity0.112 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaCancer
Sample Typeserum, plasma, tissue homogenates, cell culture supernates and other biological fluids

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