Human ACCPA(anti-cyclic citrullinated peptide antibody) ELISA Kit


Human ACCPA(anti-cyclic citrullinated peptide antibody) ELISA Kit

Cat No:HR1E9221
Price:$399/96T
Product available

  Product info

Alternative Name: ACCPA

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to anti-cyclic citrullinated peptide antibody(ACCPA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-cyclic citrullinated peptide antibody(ACCPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain anti-cyclic citrullinated peptide antibody(ACCPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of anti-cyclic citrullinated peptide antibody(ACCPA) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human ACCPA(anti-cyclic citrullinated peptide antibody) ELISA Kit
Cat NoHR1E9221
SpeciesHuman
Alternative NameACCPA
Sensitivity0.56 ng/mL
Standard100 ng/mL
Detection Range1.57-100 ng/mL
Assay Length3.5h
Research AreaAutoimmunity
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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