Human IS(serum indoxyl sulfate) ELISA Kit


Human IS(serum indoxyl sulfate) ELISA Kit

Cat No:HR1E9268
Price:$399/96T
Product available

  Product info

Alternative Name: IS; indoxyl sulfate; serum indoxyl sulfate

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to serum indoxyl sulfate(IS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to serum indoxyl sulfate(IS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain serum indoxyl sulfate(IS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of serum indoxyl sulfate(IS) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human IS(serum indoxyl sulfate) ELISA Kit
Cat NoHR1E9268
SpeciesHuman
Alternative NameIS; indoxyl sulfate; serum indoxyl sulfate
Sensitivity50 ng/mL
Standard2000 ng/mL
Detection Range31.25-2000 ng/mL
Assay Length3.5h
Research AreaKidney diseases
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids

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