Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit


Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Cat No:HR1E9273
Price:$399/96T
Product available

  Product info

Alternative Name: ACA-IgG; Anti-Cardiolipin Antibody IgG

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Cardiolipin Antibody IgG(ACA-IgG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Cardiolipin Antibody IgG(ACA-IgG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Cardiolipin Antibody IgG(ACA-IgG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Anti-Cardiolipin Antibody IgG(ACA-IgG) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit
Cat NoHR1E9273
SpeciesHuman
Alternative NameACA-IgG; Anti-Cardiolipin Antibody IgG
Sensitivity1.032 ng/mL
Standard200 ng/mL
Detection Range3.13-200 ng/mL
Assay Length3.5h
Research AreaCardiovascular biology
Sample TypeSerum, plasma, tissue homogenates and other biological fluids

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