Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit


Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit

Cat No:HR1E9321
Price:$399/96T
Product available

  Product info

Alternative Name: TC2; Transcobalamin II

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphorylated Neurofilament H(pNF-H). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphorylated Neurofilament H(pNF-H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphorylated Neurofilament H(pNF-H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Phosphorylated Neurofilament H(pNF-H) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Cat NoHR1E9321
SpeciesHuman
Alternative NameTC2; Transcobalamin II
Sensitivity29 pg/mL
Standard5000 pg/mL
Detection Range78.13-5000 pg/mL
Assay Length3.5h
Research AreaNeuro science
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.

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