Human Ret(Retinol) ELISA Kit


Human Ret(Retinol) ELISA Kit

Cat No:HR1E9339
Price:$399/96T
Product available

  Product info

Alternative Name: VA; Vitamin A; Aquasola

Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Retinol(Ret) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Retinol(Ret). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Retinol(Ret) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human Ret(Retinol) ELISA Kit
Cat NoHR1E9339
SpeciesHuman
Alternative NameVA; Vitamin A; Aquasola
Sensitivity45.1 ng/mL
Standard10000 ng/mL
Detection Range156.25-10000 ng/mL
Assay Length2h
Research AreaMetabolic pathway;Endocrinology;Hormone metabolism;
Sample Typeserum, plasma and other biological fluids

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