Chicken COL7(Collagen Type VII) ELISA Kit


Chicken COL7(Collagen Type VII) ELISA Kit

Cat No:HR1E9455
Price:$399/96T
Product available

  Product info

Alternative Name: COL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa,Dystrophic,Dominant And Recessive; Collagen Alpha-1(VII)chain

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VII(COL7). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type VII(COL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VII(COL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Collagen Type VII(COL7) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Chicken COL7(Collagen Type VII) ELISA Kit
Cat NoHR1E9455
SpeciesChicken
Alternative NameCOL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa,Dystrophic,Dominant And Recessive; Collagen Alpha-1(VII)chain
Sensitivity6.1 pg/mL
Standard1000 pg/mL
Detection Range15.63-1000 pg/mL
Assay Length3.5h
Research AreaMetabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
Sample TypeSerum, plasma, tissue homogenates and other biological fluids

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