Mouse NUCB2(Nucleobindin 2) ELISA Kit


Mouse NUCB2(Nucleobindin 2) ELISA Kit

Cat No:HR1E9851
Price:$399/96T
Product available

  Product info

Alternative Name: NUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleobindin 2(NUCB2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nucleobindin 2(NUCB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleobindin 2(NUCB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Nucleobindin 2(NUCB2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse NUCB2(Nucleobindin 2) ELISA Kit
Cat NoHR1E9851
SpeciesMouse
Alternative NameNUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin
Sensitivity0.102 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaSignal transduction;
Sample TypeSerum, plasma and other biological fluids.

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