| Gene ID | 12981 |
| Accession | P01587 |
| Alternative Name | GMCSF, Colony-stimulating factor, CSF, Csfgm, Csf2<br/>Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2) |
| Species | Mouse |
| Source | E. coli |
| Description | GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non-glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. |
| Accession | P01587 |
| Functions | The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ? 0.1 ng/mL, corresponding to a specific activity of ? 3.0 x 10^6 units/mg. |
| Formulation | Recombinant mouse GM-CSF was lyophilized from 0.2 ?m filtered PBS solution, pH 7.0. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 14 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |