Recombinant Mouse IL1A


Recombinant Mouse IL1A

Cat No.:HR2R2158
Product available

  Product info

Quantity: 100 μg

Gene Symbol: IL1A
Product Details

Gene ID 16175
Accession P01582
Alternative NameIL-1 alpha, IL1a<br/>Recombinant Mouse Interleukin-1 Alpha (IL1A)
SpeciesMouse
SourceE. coli
Description Monocytes are the main source of secreted IL-1. They express predominantly IL-1- beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
Accession P01582
Functions The ED(50) was determined by a cell proliferation assay using the murine helper T cell line, D10.G4., was found to be 10.0 pg/mL.
FormulationRecombinant mouse Interleukin-1 alpha was lyophilized from a 0.2 ?m filtered PBS solution.
Solubility A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Appearance Lyophilized Powder
Molecular Weight18
Purity>95% as determined by SDS-PAGE
Concentration < 1.0 EU/μg of recombinant protein as determined by the LAL method.
Shipping ConditionAmbient Temperature
Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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