Recombinant Mouse IL1B


Recombinant Mouse IL1B

Cat No.:HR2R2160
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  Product info

Quantity: 1.0 μg

Gene Symbol: IL1B
Product Details

Gene ID 16176
Accession P10749
Alternative NameIL-1 beta, IL1b<br/>Recombinant Mouse Interleukin-1 Beta (IL1B)
SpeciesMouse
SourceE. coli
Description Monocytes are the main source of secreted IL-1. They express predominantly IL1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
Accession P10749
Functions The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ? 2.0 pg/mL, corresponding to a specific activity of ? 5.0 x 10^8 units/mg.
FormulationMouse IL-1 beta was lyophilized from a 0.2 ?m filtered solution in PBS, pH 7.
Solubility A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Appearance Lyophilized Powder
Molecular Weight17
Purity>95% as determined by SDS-PAGE
Concentration <1.0 EU/μg of recombinant protein as determined by the LAL method.
Shipping ConditionAmbient Temperature
Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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