| Gene ID | 20307 |
| Accession | Q9Z121 |
| Alternative Name | C-C motif chemokine 8, Monocyte chemotactic protein 2, MCP2, Small-inducible cytokine A8, Mcp2, Scya8<br/>Recombinant Mouse Monocyte Chemoattractant Protein-2 (CCL8) |
| Species | Mouse |
| Source | E. coli |
| Description | Monocyte chemotactic protein (MCP-2) is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells. Also, its spectrum of action on leukocytes is similar to that of MCP-3. The leukocytes affected are monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils - differs from MCP-1 which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP-1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells. |
| Accession | Q9Z121 |
| Functions | The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/mL. |
| Formulation | Mouse MCP-2 was lyophilized from a 0.2 ?m filtered 20 mM PB solution pH 7.5, 100 mM NaCl. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 9 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |