| Gene ID | 1489 |
| Accession | Q16619 |
| Alternative Name | Recombinant Human Cardiotrophin-1, CTF1, CT1, CT-1 |
| Species | Human |
| Source | E. coli |
| Description | Cardiotrophin-1 is a pleiotrophic cytokine and is a member of the IL-6 cytokine family that also includes IL6, IL11, LIF, OSM, CLC and IL31.Full length human CT-1 shares a 79% amino acid sequence identity with murinic CT-1 and both lack a canonical signal sequence. Cardiotrophin-1 interacts with the leukemia inhibitory factor receptor (LIFR) heterodimer and the glycoprotein 130 subunit (gp130) and has the ability to induce cardiac myocyte hypertrophy. It is highly expressed in various skeletal muscles and cardiac tissue, as well as the colon, ovary and prostate, liver, and pancreas, while lower expression levels are found in the lungs, kidney, pancreas, testis, and small intestine. |
| Accession | Q16619 |
| Functions | ED50 is ? 0.5 ng/mL as determined by the dose-dependent TF-1 cell proliferation. |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PBS |
| Solubility | Reconstitute in sterile 20mM Tris, pH 8.0 at 100μg/mL (containing at least 0.1% human or bovine serum albumin). Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1?1.0% BSA. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 21 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |