| Gene ID |
681112 |
| Accession | B8K1Y1 |
| Alternative Name | IL-20<br/>Recombinant Rat Interleukin-20 (IL20) |
| Species | Rat |
| Source | E. coli |
| Description | IL-19 and IL-20 are mainly produced by activated monocytes , IL-24 is produced by Th2 cells, monocytes, and melanocytic cells , and IL-22 and IL-26 are primarily produced by activated T cells. Like IL-10, these class II cytokines signal through heterodimeric receptors. Overexpression of IL-20 in transgenic mice causes neonatal death as well as skin abnormalities, including aberrant epidermal differentiation. IL-20 selectively enhances multipotential hematopoietic progenitors in vitro and in vivo. IL-20 is preferentially expressed in monocytes18 and induces STAT 3 activation through binding to 2 types of IL-20 receptor (R) complexes, either IL- 20R1/IL-20R2 or IL-22R1/IL- 20R2 |
| Accession | B8K1Y1 |
| Functions | The ED(50) was determined by by a cell proliferation assay using human IL-20R-alpha and human IL-20R-beta co-transfected murine BaF3 pro-B cells, and was found to be less than 0.6 ng/mL, corresponding to a specific activity of 1.7 x 10^6 IU/mg. |
| Formulation | Recombinant Rat Interleukin-20 was lyophilized from a 0.2 ?m filtered PBS solution pH 7.5. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 16 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
