| Gene ID |
24835 |
| Accession | P16599 |
| Alternative Name | TNF-alpha, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnfsf2, Tumor necrosis factor <br/>Recombinant Rat Tumor Necrosis Factor Alpha (TNF) |
| Species | Rat |
| Source | E. coli |
| Description | TNF is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF-alpha while CD8(+) cells secrete little or no TNF-alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non-glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF-alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF-alpha forms dimers and trimers. |
| Accession | P16599 |
| Functions | The ED50 as determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40 ng/mL |
| Formulation | Lyophilized from a 0.2 ?m filtered PBS solution (pH 7.0) |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers |
| Appearance | Lyophilized Powder |
| Molecular Weight | 17 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
