| Gene ID | |
| Alternative Name | ply, spr1739, Pneumolysin, Thiol-activated cytolysin |
| Species | Streptococcus pneumoniae (strain ATCC BAA-255 / R6) |
| Source | Yeast |
| Functions | A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. |
| Formulation | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference |
| Appearance | Lyophilized powder |
| Purity | >85% (SDS-PAGE) |
| Storage Condition | Store at -20?C/-80?C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |