| Gene ID | N/A |
| Accession | NM_000075, NM_053056 |
| Alternative Name | CDK4: CMM3; PSK-J3; MGC14458 CyclinD1: BCL1, PRAD1, U21B31, D11S287E |
| Species | Human |
| Source | Insect cells |
| Description | CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex . CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 . |
| Accession | NM_000075, NM_053056 |
| Functions | The specific activity of CDK4/CyclinD1 was determined to be 12 nmol/min/mgas per activity assay protocol using protein substrate Rb (773-928). |
| Formulation | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. |
| Solubility | N/A |
| Appearance | Liquid |
| Molecular Weight | 57 |
| Purity | 70% - 90% |
| Shipping Condition | Dry Ice |
| Storage Condition | Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |