Recombinant Human CXCL12 (SDF-1 alpha)


Recombinant Human CXCL12 (SDF-1 alpha)

Cat No.:HR2R1304
Product available

  Product info

Quantity: 10 μg

Gene Symbol: CXCL12
Product Details

Gene ID 6387
Accession P48061
Alternative NameStromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF<br/>Recombinant Human Stromal Cell-Derived Factor-1 Alpha (CXCL12)
SpeciesHuman
SourceE. coli
Description SDF-1-alpha and SDF-1-beta are small cytokines belonging to the CXC-Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL-7 and isolated originally from a murine bone marrow stromal cell line. Human SDF-1-alpha and SDF-1-beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is a highly potent chemoattractant for mononuclear cells in vivo. In addition. SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL-3 in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.
Accession P48061
Functions Determined by its ability to chemoattract human U937 expressing CXCR4 Determined by calcium flux with human U937 cells.
FormulationLyophilized from a 0.2 ?m filtered solution in PBS, pH 7.4
Solubility A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Appearance Lyophilized Powder
Molecular Weight8
Purity>95% as determined by SDS-PAGE
Concentration <1.0 EU/μg of recombinant protein as determined by the LAL method
Shipping ConditionAmbient Temperature
Storage Condition The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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