| Gene ID | 9547 |
| Accession | O95715 |
| Alternative Name | Chemokine BRAK, MIP-2G, Small-inducible cytokine B14 |
| Species | Human |
| Source | E. coli |
| Description | Chemokine (CXC motif) ligand 14, also known as breast and kidney-expressed chemokine (BRAK), is a CXC chemokine that is a potent chemoattractant for neutrophils but not for T-cells, B-cells, monocytes or granulocytes. Mature CXCL14 has many conserved features of the CXC family and is 30% homologous to the MIP-? and MIP-? sequences, but differs in that it has an additional five amino acid sequence in between the third and fourth cysteines and a short N-terminus. It is usually highly expressed near its fibroblast source and in epidermal fibroblasts and keratinocytes of skin, but is absent from cancer cells. |
| Accession | O95715 |
| Functions | The ED50 is 0.25-1.25 ng/mL as determined by its ability to induce calcium flux in activated monocytes |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PBS |
| Solubility | Reconstitute in sterile 4mM HCl at 100μg/mL (containing at least 0.1% human or bovine serum albumin) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 9.6 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |