| Gene ID | 5196 |
| Accession | P02776 |
| Alternative Name | C-X-C motif chemokine 4, Iroplact, Oncostatin-A, CXCL4, SCYB4<br/>Recombinant Human Platelet Factor 4 (PF4) |
| Species | Human |
| Source | E. coli |
| Description | Platelet factor 4 (PF4/CXCL4) is an ELR? tetrameric, cationic chemokine that constitutes 25% of the protein in platelet alpha-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells. |
| Accession | P02776 |
| Functions | Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 5-15 μg/mL. |
| Formulation | Recombinant PF4/CXCL4 was lyophilized from a 0.2 ?m filtered concentrated (1.0 mg/mL) solution in 20 mM PB,1.5 M NaCl pH 7.4. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 8 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |