| Gene ID |
50846 |
| Accession | O43323 |
| Alternative Name | Desert Hedgehog Protein, DHH<br/>Recombinant Human Desert Hedgehog (DHH) |
| Species | Human |
| Source | E. coli |
| Description | The Desert Hedgehog protein is one of the three Hedgehog family proteins that is highly conserved and plays a role in many developmental processes in animals. DHH become biologically active following its autocatalytic cleavage. There is a high degree of residue homology between Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and DHH. Despite their individual levels of expression and unique biological roles, the Hedgehog family members all use the same signalling pathway, and therefore, may be used interchangeably for some experimental purposes. |
| Accession | O43323 |
| Functions | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 15?-45 μg/mL |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PBS with 0.2 mM DTT |
| Solubility | Reconstitute at 500 μg/mL in sterile PBS |
| Appearance | Lyophilized Powder |
| Molecular Weight | 20 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | < 1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
