| Gene ID |
N/A |
| Accession | P39905 |
| Alternative Name | GDNF, Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF<br/>Recombinant Human Glial-Derived Neurotrophic Factor (GDNF) |
| Species | Human |
| Source | E. coli |
| Description | GDNF is a disulfide-linked, homodimeric neurotrophic factor that belongs to the cystine-knot family of growth factors. Natively glycosylated, GDNF signals through a multi-component receptor system that comprises of a RET and one of the four GFR? receptors. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity Recombinant Human GDNF is a non-glycosylated, disulfide-linked homodimer (30 kDa) consisting of 135 amino acids in each subunit. |
| Accession | P39905 |
| Functions | The ED50 as determined by the proliferation of SH-SY5Y cells was found to be <10 ng/mL |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in Sodium Citrate and NaCl (pH 4.0) |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 15 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
