| Gene ID | 1437 |
| Accession | P04141 |
| Alternative Name | GMCSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, pluripoietin-alpha, GMCSF <br/>Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (CSF2) |
| Species | Human |
| Source | CHO cells |
| Description | GM-CSF is a hematopoietic growth factor that is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes following their activation by antigens or mitogens. GM-CSF stimulates the development of macrophages and neutrophils and plays a role in the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo compared to its non-glycosylated counterpart. Human and murine GM-CSF are species-specific and therefore, exhibit no cross-reactivity. Recombinant Human GM-CSF is a glycosylated, monomeric protein (contains intra-chain disulfide bonds) that migrates at approximately 25 kDa in a reducing SDS-PAGE. |
| Accession | P04141 |
| Functions | The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found be to ? 0.1 ng/mL |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PBS, pH 7.0 |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 15 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |