| Gene ID |
3606 |
| Accession | Q14116 |
| Alternative Name | IL-18, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma<br/>Recombinant Human Interleukin-18 (IL18) |
| Species | Human |
| Source | E. coli |
| Description | Also known as interferon-gamma-inducing factor (IGIF) and IL-1?, IL18 is a cytokine that bears structural similarities with members of the IL-1 family of proteins. IL18 is natively synthesized as a proform that gets processed by caspase-1 to generate the bioactive form of IL18. IL18 is one of the pro-inflammatory cytokines that is expressed by a variety of immune and non-immune cells. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL18 functions as a growth and differentiation factor for Th1 cells and is known to up-regulate the FAS ligand mediated cytotoxic activity of murine natural killer cells. IL18 also partakes in a complex regulatory circuit involved in causing cell death by apoptosis. |
| Accession | Q14116 |
| Functions | The ED(50) as determined by the dose-dependent proliferation of human PBMC co-stimulated with IL12 was found to be in the range of 5.0 ng/mL. |
| Formulation | Lyophilized from 0.2 ?m filtered solution in Tris buffer with NaCl |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 18.5 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | < 1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
