| Gene ID |
386653 |
| Accession | Q6EBC2 |
| Alternative Name | IL-31<br/>Recombinant Human Interleukin-31 (IL31) |
| Species | Human |
| Source | E. coli |
| Description | IL-31 is a recently discovered helical cytokine, belonging to the gp130/IL-6 cytokine family. The receptors for IL-6 family are type I receptors, and they share a number of common structural motifs, such as the cytokine-binding domain with two pairs of conserved cysteine residues and a WSXWS sequence motif in the extracellular domain. IL-31 is expressed preferentially by activated Th2 CD4+ T cells, signaling through a heterodimeric receptor complex composed of IL-31RA and OSMR. Recent studies in both humans and mice have suggested a link between IL-31/IL-31R expression and skin inflammation although the functional significance of endogenous IL-31/IL-31R interactions in influencing innate and adaptive immune responses remains unknown. |
| Accession | Q6EBC2 |
| Functions | The ED(50) was determined by the ability to induce STAT3 activation in U87 MG cells, and was determined to be not less than 7.0 ng/mL. |
| Formulation | Recombinant Interleukin-31 was lyophilized from a 0.2 ?m filtered PBS solution pH 7.5. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 18 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
