| Gene ID |
3565 |
| Accession | P05112 |
| Alternative Name | IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BCGF, BCDF<br/>Recombinant Human Interleukin-4 (IL4) |
| Species | Human |
| Source | CHO cells |
| Description | IL4 is produced mainly by a sub-population of activated T helper cells which also secrete IL5 and IL6. The biological activities of IL4 are mediated by a specific receptor, the extracellular domain of which is related to the receptors for EPO, IL6, and the beta chain of the IL-2 receptor. IL4 participates in several B-cell activation processes as well as of other cell types. A co-stimulator of DNA-synthesis, IL4 induces the expression of class II MHC molecules on resting B-cells, enhances both secretion and cell surface expression of IgE and IgG1, and also regulates the expression of the low affinity Fc receptor for IgE on both lymphocytes and monocytes. Pre-treatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. |
| Accession | P05112 |
| Functions | The ED(50) was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ? 0.1 ng/mL, corresponding to a specific activity of > 1.0 x 10^7 units/mg. |
| Formulation | Interleukin-4 was lyophilized from a 0.2 ?m filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM glutamic acid, pH 4.5. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 15 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
