| Gene ID | 3630 |
| Accession | P01308 |
| Alternative Name | INS |
| Species | Human |
| Source | E. coli |
| Description | As secreted heterodimeric peptide hormone produced by the pancreas, Insulin is central to regulating the metabolism of carbohydrates and fat in the body. Insulin increases cell permeability to monosaccharides, amino acids and fatty acids and accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
| Accession | P01308 |
| Functions | Activity was determined to be 30 IU/mg. |
| Formulation | Lyophilized from a 0.2 ?m filtered solution with no additives |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water. To increase solubility, a few drops of acetic acid can be added to reduce the pH; solubilize at 1-2 mg/ml in dilute acetic or HCl, pH 2?3. This solution can then be diluted into other buffers to a concentration not less than 0.1 mg/mL. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 6 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |