| Gene ID | 6357 |
| Accession | Q99616 |
| Alternative Name | Monocyte chemotactic protein 4, CCL13, NCC-1, C-C motif chemokine 13, Small-inducible cytokine A13, MCP4, NCC1, SCYA13<br/>Recombinant Human Monocyte Chemoattractant Protein-4 (CCL13) |
| Species | Human |
| Source | E. coli |
| Description | Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is member of a distinct, structurally-related subclass of CC chemokines. MCP-4/CCL13 is a major chemoattractants for eosinophils, basophils, monocytes, and T lymphocytes. The MCP protein family bind to specific G-protein-coupled receptors, initiating a signal cascade within the cell. Expression of MCP-4/CCL13 mRNA and protein is greater in the sputum, epithelium, submucosal inflammatory cells, and bronchoalveolar lavage fluid of asthmatics than in healthy individuals. Involvement of MCPs has also been demonstrated in renal inflammation and atopic dermatitis. The expression of this protein is also correlated with enhanced inflammatory immune responses during immunotherapy. The recombinant construct corresponds to the ?short-chain? of CCL13 spanning amino acids 24 ? 98. |
| Accession | Q99616 |
| Functions | Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL. |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in PB, 100mM NaCl, pH 7.5 |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 9 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |