| Gene ID | 1435 |
| Accession | P09603 |
| Alternative Name | Macrophage colony-stimulating factor 1, CSF-1, MCSF, MCSF, Lanimostim <br/>Recombinant Human Macrophage Colony Stimulating Factor (CSF1) |
| Species | Human |
| Source | E. coli |
| Description | M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B cells and T cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto-oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes. |
| Accession | P09603 |
| Functions | The ED(50) was determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is < 1.0 ng/mL, corresponding to a specific activity of > 1.0 x 10^6 units/mg. |
| Formulation | Recombinant M-CSF was lyophilized from a 0.2 ?m filtered PBS solution pH 7.0 containing 1% HSA, 3% mannitol. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 18.5 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |