| Gene ID |
4909 |
| Accession | P34130 |
| Alternative Name | NT-4, Neurotrophin-5, NT-5, Neutrophic factor 4, NTF5, NTF4<br/>Recombinant Human Neurotrophin-4 (NTF4) |
| Species | Human |
| Source | E. coli |
| Description | Neurotrophins are well-known retrograde signaling molecules that regulate differentiation and cell survival in many central and peripheral neurons. Neurotrophin-4 (NT-4) is the most recently discovered neurotrophic factor in mammals and, functionally, the least well understood. Little is known about the role of NT-4 in supporting the survival of identified classes of sensory neurons. Currently, the only available data are from cell counts of whole sensory ganglia. Mice lacking NT-4 (NT-4/) exhibit a loss of ~50% of the neurons in the nodose-petrosal and geniculate ganglia but no apparent loss of neurons in the dorsal root ganglia. |
| Accession | P34130 |
| Functions | The ED(50) was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures, and was found to be in a range of 10-50 ng/mL. |
| Formulation | Recombinant NT-4 was lyophilized from a 0.2 ?m filtered PBS solution pH 7.5. |
| Solubility | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Appearance | Lyophilized Powder |
| Molecular Weight | 14 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
