| Gene ID | N/A |
| Accession | NM_002880 |
| Alternative Name | NS5; CRAF; Raf-1; c-Raf |
| Species | Human |
| Source | Insect cells |
| Description | RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly . The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration . |
| Accession | NM_002880 |
| Functions | The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol. |
| Formulation | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. |
| Solubility | N/A |
| Appearance | Liquid |
| Molecular Weight | 63 |
| Purity | 70% - 90% |
| Shipping Condition | Dry Ice |
| Storage Condition | Store product at ?70?C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |