| Gene ID | 2208 |
| Accession | P06734 |
| Alternative Name | Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23, B cell differentiation antigen<br/>Recombinant Human C-type Lectin Domain Family 4 Member J (FCER2) |
| Species | Human |
| Source | E. coli |
| Description | CD23 is a calcium dependent transmembrane glycoprotein which is expressed in many cell types. The cytoplasmic region of the protein has two isoforms – the 'a' isoform that participates in IgE-regulated endocytosis, and the 'b' isoform, which is involved in IgE-regulated phagocytosis. It has been found that there are at least four soluble forms of the CD23 protein (sCD23) all of which are produced by the activity of metallo/cysteine proteases. In addition to binding IgE, sCD23 have been shown to promote the activation and differentiation of B-cells and stimulate the release of pro-inflammatory cytokines from monocytes. |
| Accession | P06734 |
| Functions | Measured by its ability to bind in a functional ELISA. Immobilized rhCD23 at 2 µg/mL (100 µL/well) can bind human IgE with a linear range of 0.0151 µg/mL. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS |
| Solubility | Reconstitute at 100 μg/mL in sterile PBS |
| Appearance | Lyophilized Powder |
| Molecular Weight | 19.5 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |