| Gene ID |
7471 |
| Accession | P04628 |
| Alternative Name | Proto-oncogene Int-1 homolog, Wnt-1<br/>Recombinant Human Proto-oncogene Wnt-1 (WNT1) |
| Species | Human |
| Source | E. coli |
| Description | Wnt-1 is a secreted protein that signals through the frizzled family of seven transmembrane receptors. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the up-regulation of over fifty genes. Stabilization and resulting accumulation of the intracellular signaling protein ?-catenin is an important aspect of Wnt-1 signaling. While known to play in role as a developmental protein, elevated levels of Wnt proteins have also been associated with tumorigenesis. |
| Accession | P04628 |
| Functions | Determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200ng/ml of human BMP-2. |
| Formulation | Lyophilized from a 0.2 ?m filtered solution in Tris and DTT |
| Solubility | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA) and store in working aliquots at -20 to -80?C |
| Appearance | Lyophilized Powder |
| Molecular Weight | 38.5 |
| Purity | >95% as determined by SDS-PAGE |
| Concentration | <1.0 EU/μg of recombinant protein as determined by the LAL method |
| Shipping Condition | Ambient Temperature |
| Storage Condition | The lyophilized protein is stable for at least one year from date of receipt at -70?C. Upon reconstitution, this cytokine can be stored in working aliquots at 2? - 8?C for one month, or at -20?C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles. |
