| Research Topic | Microbiology |
| Uniprot ID | P0DTC2 |
| Gene Names | S |
| Organism | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| AA Sequence | RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF |
| Expression Region | 319-541aa |
| Sequence Info | Partial |
| Source | Yeast |
| Tag Info | N-terminal 6xHis-sumostar-tagged |
| MW | 38.2 kDa |
| Lead Time | 3-7 business days |
| Distributor Price 2 | 100ug |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Biological Activity | ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ?g/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A1GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 19.60-39.42 ng/ml. ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 ?g/ml can bind human ACE2 (CSB-MP866317HU), the EC50 of SARS-CoV-2-S1-RBD protein is 31.80 - 44.69 ng/ml. ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ?g/ml can bind SARS-CoV-2-S Antibody (CSB-RA33245A0GMY), the EC50 of SARS-CoV-2-S1-RBD protein is 13.48-19.50 ng/ml. ?SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay. ?SARS-CoV-2 Spike protein RBD His/Sumostar Tag (CSB-YP3324GMY1) captured on COOH chip can bind SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with an affinity constant of 28.2nM as detected by LSPR Assay. |
| Storage Buffer | Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?. |
| Endotoxin | Not test. |