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Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)


Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)

Cat No:HR4S1172
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Type: Proteins
Product Details
Research TopicCell Biology
Uniprot IDQ9BYF1
Gene NamesACE2
OrganismHomo sapiens (Human)
AA SequenceQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
Expression Region18-740aa
Sequence InfoPartial
SourceMammalian cell
Tag InfoC-terminal hFc-tagged
MW112.5 kDa
Lead Time3-? business days
Distributor Price 2100ug
PurityGreater than 85% as determined by SDS-PAGE.
Biological Activity?Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2?g/well (2?g/ml, 100?l/well) of SARS-CoV-2 S1 protein (CSB-MP3324GMY), add 100?l/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100?l/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. ?Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5?g/well (5?g/ml, 100?l/well) of SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1), add 100?l/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100?l/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. ?Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.5?g/well (5?g/ml, 100?l/well) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1), add 100?l/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100?l/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. ?Measured by ACE2 protein (CSB-MP866317HU) binding ability in a functional ELISA. Coat the plate with 0.2?g/well (2?g/ml, 100?l/well) of ACE2 protein, add 100?l/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1) and 100?l/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific (CSB-PA160506). Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. ?The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-YP3324GMY1). ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. ?The SPR sensorgram shows the binding between ACE2 protein (CSB-MP866317HU) and SARS-CoV-2 S1-RBD protein (CSB-MP3324GMY1b1). ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
Storage BufferLyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.

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